Resumo

CARVALHO, A.; OLEASTRO, M.; NUNES, B.  e  MONTEIRO, L.. Avaliação do potencial patogénico dos genes sabA e hopZ de estirpes de Helicobacter Pylori isoladas numa população portuguesa . J Port Gastrenterol. [online]. 2006, vol.13, n.6, pp.258-266. ISSN 0872-8178.

To study the potential of sabA and hopZ genes as virulence markers for peptic ulcer disease (PUD) in H. pylori strains isolated from Portuguese patients, 106 isolated from adults (50 with PUD, 56 with non-ulcer dyspepsia) and 74 from children (21 with PUD, 53 with non-ulcer dyspepsia). Genotyping was performed by PCR and sequencing of the 5’ region of the genes was done. We observed that sabA on was significantly more often associated with gastritis only in strains isolated from children. hopZ on was more prevalent in strains from PUD, although this difference was not significant. We also found that sabA on was significantly associated with cagA, vacAs2 and oipA off genotypes in the strains isolated from children and that there was no association between any functional status of sabA and hopZ with other genes in the adult population. Finally, we compared some genotype combinations. In strains isolated from children, the risk for PUD increased when sabA off was associated with: cagA+, vacAs1 and cagA+/vacAs1. We concluded that neither sabA nor hopZ are pathogenicity markers for PUD in this Portuguese population. However, the sabA off genotype can contribute to the distinction between PUD and gastritis when associated with other virulence genes.

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