Abstract

SOUSA, Vanessa Barboza de et al. Amikacin Induces Renal Morphohistological Alterations in Wistar Rats. Arq Med [online]. 2009, vol.23, n.6, pp.205-208. ISSN 2183-2447.

The aim of this experimental study was to investigate the morphohistological effects of amikacin in rats. Male Wistar rats aged 8-12 weeks and weighing 200-250g were placed in a temperature (23±2°C) controlled room in which a 12/12h light: dark cycle was maintained. Twenty-five rats were randomly assigned to five experimental groups of five animals each that received daily treatment with 15 mg/kg of amikacin intraperitoneally. The control group was treated with 0.9% saline for 28 days. Groups I, II, III and IV were injected intaperitoneally with amikacin for 7, 14, 21 and 28 days, respectively. Renal tissues slides were stained with PAS reagent and examined light microscopy. The morphology of the PAS stained structures of the renal glomeruli was evaluated in the different experimental groups using scores. The renal morphohistology and the morphologic analysis of PAS staining were analysed statistically with the Kruskal-Wallis Test and Dunn’s Multiple Comparisons Test (5% statistical significance). The results show microscopically that kidneys in the groups treated with amikacin for 3 and 4 weeks showed marked cellular necrosis (p< 0.0001), tubular obstruction, and basal membrane interruption. This result clearly shows that amikacin administration induces renal morphohistological alterations.

Keywords : amikacin; aminoglycosides; nephrotoxicity; tubular injury.

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