SciELO - Scientific Electronic Library Online

 
vol.37 issue4Does tree environment in agro-forestry ecosystems influence the population of N2 fixing soil bacteria? author indexsubject indexarticles search
Home Pagealphabetic serial listing  

Services on Demand

Journal

Article

Indicators

Related links

  • Have no similar articlesSimilars in SciELO

Share


Revista de Ciências Agrárias

Print version ISSN 0871-018X

Abstract

MADUREIRA, Ana Margarida; DUARTE, Aida; CORREIA, Ana Isabel  and  TEIXEIRA, Generosa. Sanguisorba hybrida: pharmacognostic studies and antimicrobial activity evaluation of crude extracts. Rev. de Ciências Agrárias [online]. 2014, vol.37, n.4, pp.482-490. ISSN 0871-018X.

The genus Sanguisorba L. (Rosaceae) includes perennials that are distributed throughout the northern hemisphere. Some species of this genus are known by their medicinal interest. In the present work the pharmacognostic characterization and the potential antimicrobial activity of several extracts of Sanguisorba hybrida, endemic in Portugal, were evaluated. The leaves of the plant have been observed under microscopy techniques, tector and secretor multicellular trichomes were seen on both leaf surfaces. With histochemical tests the in situ localization of compounds was recognized and the most relevant were mucilages, phenols and terpenoids. Powdered plant material was extracted with n-hexane, dichloromethane, ethyl acetate, methanol and water. Their phytochemical survey, through TLC on silica gel plates and the proper reagents, was performed and the previous results with histochemical tests were confirmed. All extracts were tested against reference and multiresistant bacterial strains: Gram-positive (Enteroccocus faecalis, Staphylococcus aureus, Staphylococcus epidermidis and Mycobacterium smegmatis); Gram-negative (Pseudomonas aeruginosa, Salmonella typhimurium, and Klebsiella pneumoniae) and the yeast Candida albicans. The minimum inhibitory concentrations (MIC) were determined by the serial broth micro dilution method. Appropriated antibiotics were used as controls. The methanol and water extracts showed better antimicrobial activity than n-hexane, dichloromethane, ethyl acetate extracts. Gram-positive bacteria were the most sensitive and the MIC values of 3.50-1.75 µg/mL were obtained using those polar extracts against Staphylococcus aureus, including strains resistant to meticillin (MRSA).

Keywords : Sanguisorba hybrida; Rosaceae; pharmacognostic studies; antimicrobial activity.

        · abstract in Portuguese     · text in English     · English ( pdf )

 

Creative Commons License All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License