SciELO - Scientific Electronic Library Online

 
vol.33 número1Variabilidad espacial de los atributos físico-hídricos del suelo y de la productividad del cultivo de fréjol (Phaseolus vulgaris L) irrigado bajo un sistema de siembra directaInfluência do comprimento do sulco sobre a equação de infil­tração obtida pelo método dos dois pontos de Elliot & Walker índice de autoresíndice de assuntospesquisa de artigos
Home Pagelista alfabética de periódicos  

Serviços Personalizados

Journal

Artigo

Indicadores

Links relacionados

  • Não possue artigos similaresSimilares em SciELO

Compartilhar


Revista de Ciências Agrárias

versão impressa ISSN 0871-018X

Resumo

MARTINS, M. R.; SANTOS, F.; CANDEIAS, P.  e  CRUZ-MORAIS, J.. Effect of temperature, pH and Hg2+ and Pb2+ traces in dehydrogenaseand urease activities of a soil from Évora region. Rev. de Ciências Agrárias [online]. 2010, vol.33, n.1, pp.314-322. ISSN 0871-018X.

Enzyme activities are often used as in­dicator of soil quality. This study reports on dehydrogenase (EC 1.1.1) and urease (EC 3.5.1.5) activities of a soil under Olea europaea L. from Évora region. Kinetic constants Km and Vmax were determined using p-iodonitrotetrazolium chloride (INT) and urea, respectively. Effects of pH, temperature and Hg2+ and Pb2+ traces on both activities were determined. Maximal activity was obtained at pH = 8.5 and 40ºC, Km = 0.5 mM and Vmax=5,4µmol min-1 g-1 , for dehydro­genase and at pH = 10 and 37 ºC, Km = 25.7 mM and Vmax = 2.0x10-2 µmol min-1 g-1, for urease. These activities were in­hibited by different concentrations of Hg2+, but only the urease activity was in­hibited by Pb2+. Results of this study are comparable to those reported in the litera­ture for these enzymes.

Palavras-chave : Dehydrogenases; Soil enzyme; Soil quality; Urease.

        · resumo em Português     · texto em Português     · Português ( pdf )

 

Creative Commons License Todo o conteúdo deste periódico, exceto onde está identificado, está licenciado sob uma Licença Creative Commons