Resumo

BRITO, Gina; JARDIM, Roberto; SANTO, Conceição  e  COELHO, Celeste. Micropropagation of a Native Species of Porto Santo Island as a Strategy to Combat Desertification: Wild Olive as Example . Silva Lus. [online]. 2007, vol.15, n.2, pp.229-247. ISSN 0870-6352.

Micropropagation of woody plants is particularly advantageous when integrated with strategies to combat desertification and protect native species as far as it allows both preservation of germplasm in danger and large scale multiplication of plants. We describe here a protocol of micropropagation and acclimation of wild olive to Porto Santo Island that allows in the short term the integration of wild olive plants in ongoing forestation programmes. Samples of the few individuals of Olea maderensis (wild olive) existing in Madeira Archipelago were collected and a project was developed in order to preserve and propagate these individuals using OM medium modified (enriched in Fe, Mg e Mn) and supplemented with zeatin. The micropropagated plants were acclimated to greenhouse and then acclimated to conditions of Porto Santo Island, with a survival rate up to 90%. The protocol of micropropagation/acclimation developed allows the transfer of plants in a large scale to the Island. The transferred plants show good behaviour in the Island, which is expressed by the optimal average rate of elongation of 4.76 cm/month. The analysis of chlorophyll fluorescence, chlorophyll content, membrane integrity and hidric content show that the plants are not under stress. These results are an example of the use of new R&D technologies with immediate application on protection of ecosystems in danger.

Palavras-chave : Olea sp.; acclimation to field conditions; forestation; Olea sp.; vegetal biotechnology; acclimatation aux conditions du terrain; biotechnologie végétale; repeuplement forestier; Olea sp..

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