Abstract

GONCALVES, Sónia; CAIRNEY, John; OLIVEIRA, Margarida  and  MIGUEL, Célia. Identification of Differentially Expressed Genes During Embryogenesis in Maritime Pine (Pinus pinaster). Silva Lus. [online]. 2005, vol.13, n.2, pp.203-216. ISSN 0870-6352.

Differential display of Poly (A⁺) RNA isolated from zygotic embryos of maritime pine (Pinus pinaster) in 9 stages of development was used to isolate several cDNAs of differentially expressed genes during embryo development. Many cDNA sequences share low similarities to any known DNA or protein sequences but 5 transcripts are predicted to encode homologues of DNA-binding proteins, GTP-binding proteins, lipid transferases and dehydrogenases. A full-length cDNA clone was isolated for a gene (named PpAAI-LTSS 1) whose expression appears to be specific to middle stages of development. The full-length cDNA was also isolated for two clones (named PpRab1 and PpCR1) specific to an early stage of embryo development.

Keywords : pine; embryo development; gene expression.

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