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Ciência e Técnica Vitivinícola

versão impressa ISSN 0254-0223


BURKHARDT, S. L. et al. Evaluation of rootstock grapevine in vitro in conditions of stress peraluminium. Ciência Téc. Vitiv. [online]. 2008, vol.23, n.1, pp.21-27. ISSN 0254-0223.

The aluminum is the most abundant metal and the third more common element in the terrestrial crust, with approximately 7% of your total mass and in acid soils, your toxicity is the limiting factor of larger importance for the productivity of cultures in the world. In this sense, this work search to determine a method of evaluation of the behavior of rootstock grapevine ‘Paulsen 1103’ and ‘Gravesac’, in stress conditions of aluminum in vitro. The plants were maintained in a greenhouse, in controlled conditions against diseases, and submitted the irrigation with nutritious solution for grapevine. For selection of the explants, disinfestations, introduction and in vitro maintenance were used methodologies of tissue culture for the grapevine. After the disinfestations, the explants were inoculated in tubes containing 15 mL of culture medium DSD1. The cultures in vitro were transferred for growth rooms and maintained under temperature conditions of 25°C, photoperiod of 16 hours and luminous intensity of 40-45 mol.m-2.s-1. The sustentation of the explants in the liquid culture medium was previously obtained through cork disks prepared and sterilized. The experiments were maintained with pH of the solution same to 4.0 and concentrations of Al, added in the AlCl3 form, varying from 0 to 1.375 mM. After the sterilization of the culture medium, the concentrations of aluminum were added to the culture medium in a laminar camera and the pH values corrected for 4.0. Two experiments were implanted. In the first, the explants of the two varieties of grapevine rootstock were placed directly in the liquid culture medium DSD1 with different concentrations of Al, where were maintained per 60 days until the evaluation. In the second experiment, the explants were introduced in the same culture medium, in the absence of aluminum. After 30 days in growth, the plants in vitro were selected by size and submitted to the several treatments until the evaluation. The method for the evaluation of aluminum fitotoxicity effects on the grapevine culture in vitro was elaborated with base in studies about the toxicity for aluminum in nutrition solution and the selection in vitro of grapevine. The effects of the treatments were evaluated on the radicular system (number of roots and sec mass), fresh mass and sec mass of leaves. The experimental delineate used was randomized composed by six glasses, containing three plants each one, in a total of seven treatments. The rootstock grapevine ‘Gravesac’ and ‘Paulsen 1103’, present mechanisms of aluminum exclusion through elevation of pH in the culture medium. The cv. ‘Gravesac’ presented answers variation of faster pH than ‘Paulsen 1103’. Its happened variability in the answers growth of plants in vitro in stress conditions of H+ and Al+3. The development in vitro of rootstock grapevine Paulsen 1103 was superior to Gravesac for number of leaves, length of roots, foliar area and total dry weight.

Palavras-chave : tissue culture; toxicity; Vitis spp.

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